The objective of the Integrated Approaches for Testing and Assessment (IATA) Case Studies Project is to increase experience with the use of IATA by developing case studies which constitute examples of predictions that are fit for regulatory use. The aim of this project is to create common understanding of using novel methodologies and the generation of considerations/guidance stemming from these case studies. This case study was developed by the Health and Environmental Sciences Institute (HESI), Canada, United Kingdom, United States, Business at OECD (BIAC) for illustrating practical use of IATA and submitted to the 2023 review cycle of the IATA Case Studies Project. This IATA provides examples to aid evaluators in the collection, generation, evaluation, and integration of multiple lines of evidence (LoE) for clear and transparent decision-making within defined problem contexts. This IATA includes guidance for data collection and generation from publicly available databases and models that can be readily used for data poor and relatively data rich chemicals. Three illustrative case studies representing both data poor and data rich chemicals are presented to illustrate the applicability of the IATA for Bioaccumulation assessment.